Itoh a, saitoh t, tani k, uchigaki m, sugimoto y, yamada j, nakajima h, ohshiro h, sun sj, tanahashi t 2011 bisbenzylisoquinoline alkaloids from nelumbo nucifera. Further studies revealed that three bisbenzylisoquinoline alkaloids neferine, liensinine and isoliensinine of lotus nelumbo nucifera gaertn. Prasath manogaran, narasimha murthy beeraka and viswanadha vijaya padma affiliation. Pdf benzylisoquinoline alkaloids biosynthesis in sacred lotus. Benzylisoquinoline alkaloids bias, mostly within the aporphine and bisbenzylisoquinoline structural categories, are among the main bioactive. Current advances in the metabolomics study on lotus seeds. Iamshaman sells both, and they say that both are psychoactive, and i dont know anymore which one i should be looking for.
A new lipid and other constituents from the rhizomes of nelumbo nucifera. Download citation bisbenzylisoquinoline alkaloids from nelumbo nucifera from the embryos of the seeds of nelumbo. As an important ornamental plant with beautiful flowers and pleasant fragrance, lotus has numerous common names e. Nelumbo nucifera gaertn nymphaeaceae is a wellknown medicinal plant in ancient medical sciences. The sacred water lotus has been used in the orient as a medicinal herb for well over 1,500 years. Nelumbo nucifera rhizome also improves memory function by enhancing neurogenesis in the dentate gyrus of the rat hippocampus 11, 12, and procyanidins isolated from the nelumbo nucifera seedpod ameliorate scopolamineinduced memory impairment by inhibiting ache activity.
Pdf benzylisoquinoline alkaloids biosynthesis in sacred. S1 isolation and characterization of omethyltransferases involved in benzylisoquinoline alkaloids biosynthesis in sacred lotus nelumbo nuciferaivette m. The structures of the new alkaloids were determined mainly by spectroscopic methods. Translational research lab, department of biotechnology, bharathiar university, coimbatore, tamil nadu, translational research lab, department of. Bisbenzylisoquinoline alkaloids table 7 are characterized by having the benzylisoquinoline skeleton dimerized by ether linkages or carboncarbon bonds. Isolation and characterization of twoomethyltransferases involved. Mainly, bisbenzylisoquinoline alkaloids isolated from nelumbo nucifera nymphaeaceae are being used widely in tradi tional forms of medicine in ayurveda of. Ethnomedicinal uses and pharmacological plants journal. Various parts of nelumbo nucifera have been reported to exhibit effects associated with alzheimers disease. Nelumbo nucifera lotus is a wellknown edible plant widely cultivated in asia. Young bae seo a professor of the college of oriental medicine, daejeon university, daejeon, korea and has been deposited at the. Nelumbo nucifera seeds contain variety of phytochemicallike saponins, alkaloids, polyphenolics, and carbohydrates supporting significant antioxidant activity of hann 21. The embryo of the nelumbo nucifera seed was obtained from wildlife genetic resources center at national institute of biological resources nibr. Cobalt chloride cocl 2 was known to mimic hypoxic condition.
Mode of action analyses of neferine, a bisbenzylisoquinoline. Neferine, a bisbenzylisoquinoline alkaloid, isolated from nelumbo nucifera has a wide range of biological activities. Cognitive enhancing and neuroprotective effect of the embryo. Neferine is an isoquinoline alkaloid isolated from seed embryos of nelumbo nucifera gaertn, which has a variety of biological activities. Isolation and identification of a tribenzylisoquinoline. Although more than 30 bias belonging to the 1benzylisoquinoline, aporphine, and bisbenzylisoquinoline. Lotus nelumbo nucifera is an aquatic plant, belonging to the monogeneric family. Food related to lotus is popular in east asia, especially china. Lotus belongs to the genus nelumbo, the sole member of the nelumbonaceae family. Research article cognitive enhancing and neuroprotective. Neferine and isoliensinine from nelumbo nucifera induced. Cognitive enhancing and neuroprotective effect of the. Bisbenzylisoquinoline alkaloid an overview sciencedirect.
Pdf bisbenzylisoquinoline alkaloids of lotus nelumbo nucifera. Apparently its not actually blue, but pink andor purple. The genus nelumbo is represented by only two species, nelumbo nucifera and nelumbo lutea. Nelumbo nucifera seeds contain variety of phytochemicallike saponins, alkaloids, polyphenolics, and carbohydrates supporting significant antioxidant activity of hann. Benzylisoquinoline alkaloids biosynthesis in sacred. Micellar electrokinetic chromatography using a cationic surfactant for rapid separation and determination of bisbenzylisoquinoline alkaloids from embryo of the seed of nelumbo nucifera gaertn. It is an ornamental plant and an important economical crop in asian countries, especially in china.
Research article cognitive enhancing and neuroprotective effect of the embryo of the nelumbo nucifera seed eunsilkim, 1 jinbaeweon, 2 borayun, 2 jiwoolee, 2 minryeeom, 2 kyoungheeoh, 1 andchoongjema 2,3 biological and genetic resources utilization division, nationa l institute of biological resources, hwangyeongro, seogu. We attempted to ascertain if bisbenzylisoquinoline alkaloids, liensinine and isoliensinine from nelumbo nucifera gaertner have antidepressant. Serotonergic mechanisms are involved in antidepressant. Isoliensinine induces apoptosis in triplenegative human. Hiv bisbenzylisoquinoline alkaloids such as nuciferine, liensinine, negferine and isoliensinine have also been isolated from the leaves of n. Under favorable circumstances the seeds of this aquatic perennial may remain viable for many years, with the oldest recorded lotus germination being from seeds 1,300. Benzylisoquinoline alkaloids bias, mostly within the aporphine and bisbenzylisoquinoline structural categories, are among the main bioactive constituents in the plant. Analysis of isoquinoline alkaloid composition and wound. Synthesis and pharmacological activity of alkaloids from.
There is a globally acknowledged demand for a broader, safer and also cheaper repertoire for the treatment of human immunodeficiency virus hiv infection. From the embryos of the seeds of nelumbo nucifera, three bisbenzylisoquinoline alkaloids, nelumboferine and nelumborines a and b, were isolated along with four known compounds, neferine, liensinine, isoliensinine and anisic acid. Jul 29, 2015 isoliensinine, liensinine and neferine are major bisbenzylisoquinoline alkaloids in the seed embryo of lotus nelumbo nucifera and exhibit potential anticancer activity. Molecules free fulltext benzylisoquinoline alkaloids. Effects of flower extract from lotus nelumbo nucifera on hypoglycemic and hypolipidemic in streptozotocininduced diabetic rats supasorn sakuljaitrong, nopparat buddhakala, sanong chomko and chusri talubmook.
This genus consists of two species, nelumbo nucifera asia, australia, russia and nelumbo lutea north america xue et al. Cytotoxic bisbenzylisoquinoline alkaloids from the roots of cyclea racemosa. It is noteworthy that different organs of this plant are used in traditional herbal medicine or folk recipes to cure different diseases and to relieve their corresponding symptoms. The embryo of the nelumbo nucifera seed sample was authenticated by dr. Isoliensinine, liensinine and neferine are major bisbenzylisoquinoline alkaloids in the seed embryo of lotus nelumbo nucifera and exhibit potential anticancer activity. Phytochemical profile and biological activity of nelumbo nucifera. The cytoprotective and anticancer potential of bisbenzylisoquinoline alkaloids from nelumbo nucifera volume. Bisbenzylisoquinoline alkaloids of lotus nelumbo nucifera.
Neferine is a bis benzylisoquinoline alkaloid extracted. Neferine, a bisbenzylisoquinoline alkaloid isolated from the green seed embryos of lotus nelumbo nucifera gaertn, has been previously shown to have various anticancer effects. Isolation and characterization of two omethyltransferases. Nelumbo nucifera is the species of lotus sacred to both hindus and buddhists. Subsequently, the alkaloids in lotus plumule products were. Bisbenzylisoquinoline alkaloids of lotus nelumbo nucifera gaertn. Translational research lab, department of biotechnology, bharathiar university, coimbatore, tamil nadu, translational research lab, department of biotechnology. Although it has been suggested that these alkaloids are synthesized in the leaf and then transported. In addition, mechanical wounding was shown to induce signi. For example, nelumbo nucifera seed protects the mouse embryonic fibroblast cells by inhibiting h 2 o 2induced cytotoxicity, and nelumbo nucifera semen improves scopolamineinduced dementia by inhibiting acetylcholinesterase ache.
Nelumbo nucifera, nelumbonaceae, bisbenzylisoquinoline. Antihiv benzylisoquinoline alkaloids and flavonoids from. Bisbenzylisoquinoline alkaloids are known to be produced via n methylcoclaurine, which is catalyzed by the p450 enzyme 47, 48. In vitro 6omt, 7omt and 4omt activity screening of nnomts. The alkaloids of sacred lotus exhibit promising anticancer, antiarrhythmic, antihiv, and. Structure of lotusine, a new watersoluble quaternary base. In the present scenario, the isolated alkaloids from seeds, stem, and plumule of n. Sacred lotus nelumbo nucifera is an ancient aquatic plant of medicinal value because of antiviral and immunomodulatory activities linked to its constituent bias. It is the normal pedestal for divine figures in buddhist art and hindu art, and often seen in jain art. Nelumbo nucifera, also known as indian lotus, sacred lotus, bean of india, egyptian bean or simply lotus, is one of two extant species of aquatic plant in the family nelumbonaceae. Pharmacological activity of nelumboferine, stereoisomers of neferine, liensinine, isoliensinine, and omethylneferine were evaluated.
Bisbenzylisoquinolinetype alkaloids may be synthesized mainly in lamina and then transported into embryo via latex through phloem translocation. Nelumbo nucifera is widely distributed in southeast asia. Bisbenzylisoquinoline alkaloids from nelumbo nucifera. Wang, rapid determination of three alkaloids from lotus plumule in human serum using an hplcdad method with a short monolithic column, food chem. Many chemical constituents have been isolated till the date. Nymphaeaceae is a potential aquatic crop grown and consumed throughout asia. Information about psychoactive lotuslily species including basics, effects, dosage, history, legal status, photos, research, media coverage, and links to other resources.
Furukawa h, yang th, lin tj 1965 on the alkaloids of nelumbo nucifera gaertn. The sacred lotus nelumbo nucifera phytochemical and. Nelumbo, alkaloids, bioactive components, mechanical wounding. In the present study, we evaluated the effect of neferine in terms of pglycoprotein pgp inhibition via in vitro cytotoxicity assays, r123 uptake assays in drugresistant cancer cells, in silico molecular docking.
So really its just a water lotus, not a blue lotus. Food and agricultural immunology 2019, 30 1, 878896. The bisbenzylisoquinoline alkaloids are represented by over 225 different compounds which have been isolated from plants in 14 different plant families guha et al. Almost all parts of nelumbo nucifera are edible, and in many asian countries it was found in the recipe of food. Inhibitory activities of antihiv benzylisoquinoline. Visualization and detection of nnomt1nnomt5 recombinant proteins. Isolation and characterization of omethyltransferases involved in benzylisoquinoline alkaloids biosynthesis in sacred lotus nelumbo nucifera ivette m. As a homology of traditional chinese medicine and food, the green embryo of nelumbo nucifera seeds mainly contain three structurerelated bisbenzylisoquinoline alkaloids. Neferine, a bisbenzylisoquinoline alkaloid, ameliorates. Lotus nelumbo nucifera is an aquatic plant, belonging to the monogeneric family nelumbonaceae. Nymphaeaceae, also known as sacred lotus, is a well known medicinal plant.
Phytochemical profile and biological activity of nelumbo. Their diversity is due to the different substituents on the aromatic rings and the number and nature of the bonds between the btiq units, sometimes leading to asymmetric centers at c1 and c1 21 they are biosynthetically obtained from. The aim of the present study was to evaluate the effect of ens on cognitive impairment induced by scopolamine and its potential neuroprotective effect against glutamateinduced cytotoxicity in ht22 cell and to investigate the underlying mechanisms. Benzylisoquinoline alkaloids biosynthesis in sacred lotus ncbi.
Alkaloids from embryo of the seed of nelumbo nucifera. Isolation and characterization of omethyltransferases. Bisbenzylisoquinoline alkaloids accumulate predominantly in the seed embryo of n. Nelumbo nucifera actually a lotus, perhaps called the sacred water lotus. Pdf micellar electrokinetic chromatography using a cationic. Benzylisoquinoline alkaloids bias are a major class of plant metabolites with many pharmacological benefits.
Bisbenzylisoquinoline alkaloid, nelumboferine which was recently isolated from the embryo of nelumbo nucifera, and stereoisomers of neferine, which is a major alkaloid of the embryo of n. Abstract this study was aimed to investigate an acute toxicity and effects of nelumbo nuciferaflower extract nnfe on hypergl ycemic. The occurrence of neoliensinine gives interesting information about the biosynthesis of tribenzylisoquinoline alkaloids in nelumbo nucifera. Bisbenzylisoquinoline alkaloids journal of natural products. Digital gene expression analysis provides insight into the. Inhibitory effects of three bisbenzylisoquinoline alkaloids. Ens 3, 10, 30, and 100 mgkg, scopolamine 1 mgkg, and donepezil 1 mgkg were administered to mice during a test period. For example, alkaloids from nelumbo nucifera, liensinine, isoliensinine and neferine exert the anticancer activity through enhanced ros generation, activation of map kinases, followed by induction of autophagy and apoptotic cell death. It is an ornamental plant and an important economical crop and has been cultivated for thousands of years throughout asia for its beautiful flowers and edible rhizomes and seeds. Using mice, the forced swimming test fst was carried out after treatment with liensinine, isoliensinine and neferine. Pdf micellar electrokinetic chromatography using a. In a structureactivity relationship study, other benzylisoquinoline, aporphine, and bisbenzylisoquinoline alkaloids, including liensinine 14, negferine 15, and isoliensinine 16, which were previously isolated from the leaves and embryo of nelumbo nucifera, were evaluated for antihiv activity. Neferine, a bisbenzylisoquinoline alkaloid, offers. In asian art a lotus throne is a stylized lotus flower used as the seat or base for a figure.
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